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Compounded ACTH preparations for ACTH stimulation tests in dogs
The ACTH stimulation test is the most reliable test to evaluate the cortisol production of the adrenal glands when an iatrogenic hyperadrenocorticism is suspected. There are different protocols available which either measure cortisol levels 60 or 90 minutes after ACTH injection. This brandnew study gives important news: Different compounded ACTH preparations give different results after 60 minutes!

Serum cortisol concentrations were measured in five healthy dogs in response to five adrenocorticotropic hormone (ACTH) preparations.

Cortisol concentrations were similar at time 0 (pre-ACTH) and at 30 and 60 minutes after injection of all forms of ACTH.

However, at 90 and 120 minutes post-ACTH, serum cortisol concentrations were significantly lower following injection of two compounded forms of ACTH.

The data showed that injection of four compounded forms of ACTH caused elevations in serum cortisol concentrations of a similar magnitude as cosyntropin in samples collected 60 minutes after administration; but concentrations at later times varied, depending on the type of ACTH used.



Source: Robert J. Kemppainen, Ellen N. Behrend, Katherine A. Busch (2005): Use of Compounded Adrenocorticotropic Hormone (ACTH) for Adrenal Function Testing in Dogs. In: Journal of the American Animal Hospital Association 41:368-372 (2005)




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