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Atopy patch test reactions in high-IgE beagles
Intradermal skin test was thought to be the `gold standard` in identifying the causing allergens in dogs with atopic dermatitis. Like in human medicine, also diagnostic trials with patch tests in dogs are published from time to time. How are the results of this test with different sources and concentrations of house dust mites? A very interesting question!

Protocols for atopy patch testing (APT) were evaluated on six high-IgE dogs sensitized to house dust mites (HDM) using various concentrations and sources of HDM.

Two sources of HDM were compared: Heska slurry and four concentrations of Greer HDM.

Saline was used as a negative control.

Patches were removed after 48 h and the sites evaluated at 0, 6, 24, 48, 72 and 96 h for erythema, macules, papules and pustules.

Each sign was scored from 0 to 3 (0 = absent and 3 = severe).

Total score was used for analysis. Mean total scores significantly increased for both Greer and Heska HDMs from 6 h, peaking at 48 h for G100 (100 mg mL1), G300 and G668, and at 72 h for Heska and G31.25.

Across all times, Heska HDM scores were significantly higher than those of G31.25 with the largest difference at 96 h.

Heska scores, however, were significantly lower than those of other Greer concentrations (G100, G300 and G668) particularly at 96 h.

No reactions were noted at saline sites.

It is concluded that Greer–HDM at 100 mg mL1 is the most suitable concentration for APT in dogs because it induces reactions comparable if not higher than more concentrated HDM preparations.



Source: MARSELLA, ROSANNA, NICKLIN, CONNIE & LOPEZ, JENNIFER (2005): Atopy patch test reactions in high-IgE beagles to different sources and concentrations of house dust mites. In: Veterinary Dermatology 16 (5), 308-314.





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SMALL ANIMAL PRACTICE

Reference intervals for blood parameters in Shetland Sheepdogsmembers
Several breeds have physiological peculiarities that induce variations in reference intervals (RIs) compared with the general canine population. Shetland sheepdogs (SSs) are reported to be more predisposed to different diseases (eg, hyperlipidemia, gallbladder mucocele, and hypothyroidism). Consequently, a breed‐specific approach is more often required. Thus, the aim of this study was to determine whether the RIs of the general canine population could be applied to that of SSs, and to generate breed‐specific RIs, where appropriate.

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