Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Privacy Policy  
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Equine /     
 
Equine Culicoides Hypersensitivity: Update of tests
Equine Culicoides Hypersensitivity still remains one of the most important (skin) diseases in horses, affecting many breeds in various countries. Still there are controversies in the diagnosis. How reliable are serum tests, and are intradermal skin tests really superior to them? An interesting brandnew study from Italy!

Intradermal tests were carried out on 18 horses with clinical signs of Culicoides hypersensitivity (CHS) and 23 horses without clinical signs of CHS, and sera from these horses were analysed by SDS-PAGE and Western blotting (W-B).

Intradermal injections of 0.1 ml of 25 ìg/ìl sterile Culicoides extract, 0.1 ml of 1 : 10 000 histamine (positive control) and 0.1 ml of physiological saline (negative control) were made in the dermis of the middle region of the neck.

Analysis of reactions indicated that a 1 cm wheal and a skinfold thickness >10% at 24 h represented a valid cut-off between horses with and without CHS.

In these conditions the test, even in winter when clinical signs were absent, had 100% sensitivity and specificity.

The W-B was performed after running Culicoides extract on a 12% polyacrylamide gel.
The test revealed the presence of several bands with molecular weight ranging from 6 to 200 kDa.

In particular, a band of 65 kDa was predominantly found in hypersensitive horses by using an anti-IgE antibody while in normal horses the same band was mainly detected by using an anti-IgG antibody.

Our results demonstrated that the skin test is a valid diagnostic test, with high sensitivity and specificity and that the band of about 65 kDa probably corresponds to the allergen involved in the pathogenesis of CHS.



Source: Ferroglio, E., Pregel, P., Accossato, A., Taricco, I., Bollo, E., Rossi, L. & Trisciuoglio, A. (2006): Equine Culicoides Hypersensitivity: Evaluation of a Skin Test and of Humoral Response.
Journal of Veterinary Medicine Series A 53 (1), 30-33.





Tell a friend   |   Print version   |   Send this article

EQUINE

Limbal squamous cell carcinoma in a Rocky Mountain Horse and geneticsmembers
One privately owned Rocky Mountain Horse and 84 Rocky Mountain Horses screened for allelic frequency were enrolled in this study. The objective: To document a case of limbal squamous cell carcinoma (SCC) in a Rocky Mountain Horse stallion determined to be homozygous for the genetic risk factor (DDB2 c.1013C>T) strongly associated with the disease in Haflinger and Belgian horses, and to determine the frequency of this allele in a larger population of Rocky Mountain Horses.

  • Subconjunctival bupivacaine, lidocaine, and mepivacaine and the corneal sensitivity in horsesmembers
  • Clodronate in horses with lamenessmembers
  • Valacyclovir in horses with equine multinodular pulmonary fibrosismembers
  • Radiculopathy associated with forelimb lameness in horsesmembers
  • Biological variations of routine blood parameters in horsesmembers
  • Prognosis for horses with deep digital flexor tendon injury after penetrating woundmembers
  • Possible maternal effect for the pace trait of horsesmembers
  • Flecainide on induced atrial fibrillation in horsesmembers
  • Sonographic technique to identify manica flexoria tears in horsesmembers
  • Novel prototype dynamic laryngoplasty system on arytenoid abductionmembers
  • Lipid peroxidation biomarkers in equine neuroaxonal dystrophymembers
  • Eosinophils of the horse: an updatemembers


  • [ Home ] [ About ] [ Contact / Request ] [ Privacy Policy ]

    Copyright © 2001-2018 VetContact GmbH
    All rights reserved