Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Privacy Policy  
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Small Animal Practice /     
 
Lineage differentiation of canine lymphoma/leukemias
Generalized or multisystemic lymphomas belong to the most frequent malignant neoplasias in dogs. In this very interesting study, multiparameter flow cytometry analysis and specific cluster differentiation (CD) molecules were used to determine the expression profiles of B- and T-cell antigens on lymph node preparations from 59 dogs with generalized or multisystemic lymphoma - and a diagnostic marker for malignancy was found.

Lymph node samples from 11 healthy dogs were labeled to validate the specificity of antibodies and to formulate guidelines for interpretation of the results obtained from lymphoma samples.

In normal lymph nodes, T-lymphocytes expressing CD3, CD4, or CD8 beta represented 59+/-11%, 43+/-8%, or 16+/-5% of the total cells, whereas B-lymphocytes expressing either CD21 or surface IgM (IgM) represented 37+/-9% or 14+/-5%, respectively.

Small lymphocytes could be distinguished from large lymphocytes by forward light scatter.

Of the patient samples 29 different breeds were represented with Golden and Labrador retriever being the most common.

The lymphoma samples segregated into three groups based on CD antigen expression.

Thirty cases predominantly expressed one or more combinations of CD79a, IgM, and CD21 representing a B-cell lineage. Three B-cell cases also expressed the stem cell antigen, CD34.

Sixteen cases expressed one or more combinations of CD3, CD4, and CD8 consistent with a T-cell lineage and CD3+CD4+CD8--phenotype was the most common.

Thirteen cases showed a mixed expression profile for T- and B-cell antigens and in three cases CD14 was highly expressed.

Clinical response was poorest for T-cell lymphomas.

Leukemic states occurred in all three phenotypes; but mixed cell cases had the greatest proportion. Dual immunofluorescence staining confirmed co-expression of T-cell (CD3) and B-cell antigens (CD79a or CD21) on neoplastic lymphocytes of six mixed cell cases.
In one mixed cell case, dual immunostaining identified lymphocyte populations that stained mutually exclusive for CD79a and CD3.
Six mixed cell lymphomas tested by PCR showed clonality for rearranged antigen receptor.
Four cases that were CD79a+CD3+ had TCRgamma chain gene rearrangements, whereas two cases that were CD3+CD8+CD21+ had Ig heavy chain rearrangement.
One case expressing multiple CD molecules (CD3+CD8+CD21+CD14+) was PCR negative for both Ig and TCRgamma gene rearrangement and could not be classified into a B- or T-cell lineage.

We show for the first time co-expression of B- and T-cell markers on lymphoma cells that had specific T- or B-cell gene rearrangements.

These findings suggest that aberrant CD molecule expression is not an uncommon finding in canine lymphomas and is a useful diagnostic marker for malignancy.


Source: Wilkerson MJ, Dolce K, Koopman T, Shuman W, Chun R, Garrett L, Barber L, Avery A. (2005): Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. In: Vet Immunol Immunopathol. 2005 Jul 15;106(3-4):179-96.





Tell a friend   |   Print version   |   Send this article

SMALL ANIMAL PRACTICE

Reference intervals for blood parameters in Shetland Sheepdogsmembers
Several breeds have physiological peculiarities that induce variations in reference intervals (RIs) compared with the general canine population. Shetland sheepdogs (SSs) are reported to be more predisposed to different diseases (eg, hyperlipidemia, gallbladder mucocele, and hypothyroidism). Consequently, a breedâ€specific approach is more often required. Thus, the aim of this study was to determine whether the RIs of the general canine population could be applied to that of SSs, and to generate breedâ€specific RIs, where appropriate.

  • Metastasis of a well differentiated perianal gland tumor
  • Punica granatum associated with hepatotoxicosis in cattlemembers
  • Toceranib phosphate (Palladia®) in canine gastrointestinal stromal tumorsmembers
  • Radioactive iodine uptake in hyperthyroid cats after rh-TSHmembers
  • Hypoechoic tissue changes in dogs with malignant prostatic lymphomamembers
  • Emphysematous gastritis in dogs and catsmembers
  • Primary pulmonary histiocytic sarcoma in dogsmembers
  • Determining prognosis in canine sepsis members
  • Correlation of plasma and tear glucose, creatinine and urea nitrogen in catsmembers
  • Perineal hernias in dogs - always a bilateral problem?members
  • Pharmacokinetic of gabapentin in catsmembers
  • Follicular development of canine ovaries stimulated by eCG plus hCGmembers


  • [ Home ] [ About ] [ Contact / Request ] [ Privacy Policy ]

    Copyright © 2001-2018 VetContact GmbH
    All rights reserved