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Australian strains of porcine circovirus types 1 and 2 - genetic characterisation
As post-weaning multi-systemic wasting syndrome (PMWS) has not been identified within Australia, to determine if the absence of disease was associated with genetic differences between the strains of porcine circovirus (PCV) present in Australia and those from countries in association with PMWS. Is there any difference? An interesting question...

Pig tissues were obtained from weaned pigs found dead or presenting with clinical signs of illthrift and also from neonatal pigs with congenital tremors and used as a source of virus DNA for sequence analysis.

DNA was extracted from the tissues and PCV detected by polymerase chain reaction (PCR). PCR with PCV type-specific primers was used to amplify the entire genome from selected tissues. The genomes of three strains of PCV1 and seven strains of PCV2 from three Australian states were sequenced and subjected to phylogenetic analysis using standard procedures.

Results: The three Australian PCV1 strains had 98 to 99% nucleotide identity to strains in other countries and the seven Australian PCV2 strains had 94 to 99% identity to PCV2 strains in other countries where PMWS has occurred.

Six of the seven Australian PCV2 strains were genetically similar to each other, while the seventh was more distantly related.

There were no consistent differences in the predicted amino acid sequence of the Australian strains of PCV2 and strains associated with PMWS in other countries.

Conclusion: There were no consistent differences between Australian strains of PCV and those that have been associated with PMWS in other countries and it appears likely that other factors are responsible for the absence of PMWS in Australia.


Source: Muhling, J, Raye, WS, Buddle, JR & Wilcox, GE (2006): Genetic characterisation of Australian strains of porcine circovirus types 1 and 2. In: Australian Veterinary Journal 84 (12), 421-425.




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SWINE PRACTICE

Beta hydroxy beta methyl butyrate and the muscle fibre composition in growing pigsmembers
The aim of this recently online published study was to investigate the effects of excess leucine (Leu) vs. its metabolites α‐ketoisocaproate (KIC) and β‐hydroxy‐β‐methyl butyrate (HMB) on Leu metabolism, muscle fibre composition and muscle growth in growing pigs. Thirty‐two pigs with a similar initial weight (9.55 ± 0.19 kg) were fed 1 of 4 diets for 45 days: basal diet, basal diet + 1.25% L‐Leu, basal diet + 1.25% KIC‐Ca, basal diet + 0.62% HMB‐Ca. The results are very promising!

  • L-carnitine as a feed additive in pigs and poultrymembers
  • PCV-3 in pig serum samples from Spainmembers
  • Oral fluid and plasma oxytetracycline concentrations after i.m. administration in pigsmembers
  • Novel highly pathogenic porcine reproductive and respiratory syndrome virus in Chinamembers
  • Calibration of the TonoVet and Tono-Pen Vet tonometers in pigs´ eyesmembers
  • Measurement of serum cobalamin and folate concentrations in pigs with commercially assaysmembers
  • Commercially available immunoassays for serum cobalamin and folate in pigsmembers
  • Ultrasound-guided vessel catheterization in adult pigsmembers
  • Association between porcine cytomegalovirus infection and PMWSmembers
  • Suitable antibiotic therapy to treat swine brucellosismembers
  • Pancuronium versus vecuronium in pigs members
  • Haemodynamic effects of pancuronium and vecuronium in pigsmembers


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