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Australian strains of porcine circovirus types 1 and 2 - genetic characterisation
As post-weaning multi-systemic wasting syndrome (PMWS) has not been identified within Australia, to determine if the absence of disease was associated with genetic differences between the strains of porcine circovirus (PCV) present in Australia and those from countries in association with PMWS. Is there any difference? An interesting question...

Pig tissues were obtained from weaned pigs found dead or presenting with clinical signs of illthrift and also from neonatal pigs with congenital tremors and used as a source of virus DNA for sequence analysis.

DNA was extracted from the tissues and PCV detected by polymerase chain reaction (PCR). PCR with PCV type-specific primers was used to amplify the entire genome from selected tissues. The genomes of three strains of PCV1 and seven strains of PCV2 from three Australian states were sequenced and subjected to phylogenetic analysis using standard procedures.

Results: The three Australian PCV1 strains had 98 to 99% nucleotide identity to strains in other countries and the seven Australian PCV2 strains had 94 to 99% identity to PCV2 strains in other countries where PMWS has occurred.

Six of the seven Australian PCV2 strains were genetically similar to each other, while the seventh was more distantly related.

There were no consistent differences in the predicted amino acid sequence of the Australian strains of PCV2 and strains associated with PMWS in other countries.

Conclusion: There were no consistent differences between Australian strains of PCV and those that have been associated with PMWS in other countries and it appears likely that other factors are responsible for the absence of PMWS in Australia.


Source: Muhling, J, Raye, WS, Buddle, JR & Wilcox, GE (2006): Genetic characterisation of Australian strains of porcine circovirus types 1 and 2. In: Australian Veterinary Journal 84 (12), 421-425.




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SWINE PRACTICE

Oral fluid and plasma oxytetracycline concentrations after i.m. administration in pigsmembers
The penetration of oxytetracycline (OTC) into the oral fluid and plasma of pigs and correlation between oral fluid and plasma were evaluated after a single intramuscular (i.m.) dose of 20 mg/kg body weight of long-acting formulation. An important study regarding one of the most popular long-acting antibiotics in pigs.

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  • Calibration of the TonoVet and Tono-Pen Vet tonometers in pigs´ eyesmembers
  • Measurement of serum cobalamin and folate concentrations in pigs with commercially assaysmembers
  • Commercially available immunoassays for serum cobalamin and folate in pigsmembers
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  • Pancuronium versus vecuronium in pigs members
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  • Exposure of pigs to lead from contaminated zinc oxide members
  • Conjugated Linoleic Acid and Growth Performance and Losses of Pigletsmembers
  • Introduction of PRRSV via Boar Semen into a PRRSV-Free Countrymembers


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