Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Swine Practice /     
 
Pregnancy diagnosis in miniature pigs by fecal ELISA
Miniature pigs are more and more seen as pets in different countries. A Japanese group evaluated a new fecal ELISA test from pregnancy diagnosis at day 27-29.

The objectives of the present study were to measure oestrone derivatives [oestrone, oestrone sulphate (E1S) and oestrone glucuronide] in sow faeces by direct enzyme-linked immunosorbent assay (ELISA) and to explore the changes of oestrone derivative profile in faeces of miniature pig for demonstrating the possibility of pregnancy diagnosis.

Faecal samples (1.5 g) were put into 6 ml of buffer, shaken and centrifuged. Then supernatant was added to the wells of multi-plate without extraction and incubated with anti-oestrone antibody and horseradish peroxidase-labelled oestrone. Standard solution was prepared at various concentrations of E1S. Sensitivity was estimated as 0.035 ng/ml (0.14 ng/g). Intra- and inter-assay coefficient variations were 3.5-7.7% and 10.9-15.3%, respectively. When 1-5 ng/ml E1S were added to a faecal solution, recovery rates ranged between 80.0 and 103.3%. There is a temporal increase in the E1S equivalent concentration of miniature pig faeces from day 25 to 31 after mating. From day 35 to 70, the E1S equivalent concentrations remained low. Thereafter its concentrations increased again towards farrowing.

On day 27 and 29 after mating pregnancy diagnosis by the faecal E1S equivalent concentrations agreed with the results of farrowing (seven of seven animals).

These results suggest that the present direct ELISA is practical and suitable as a routine assay for measuring the faecal concentration of oestrone derivatives and that this assay might be usable for pregnancy diagnosis in sows at day 27-29.


Source: N Isobe and T Nakao (2004): Pregnancy Diagnosis in Miniature Pig by Direct ELISA of Oestrone Derivatives in Faeces. In: Reproduction in Domestic Animals 39 (1), 48-51.





Tell a friend   |   Print version   |   Send this article

SWINE PRACTICE

Oral fluid and plasma oxytetracycline concentrations after i.m. administration in pigsmembers
The penetration of oxytetracycline (OTC) into the oral fluid and plasma of pigs and correlation between oral fluid and plasma were evaluated after a single intramuscular (i.m.) dose of 20 mg/kg body weight of long-acting formulation. An important study regarding one of the most popular long-acting antibiotics in pigs.

  • Novel highly pathogenic porcine reproductive and respiratory syndrome virus in Chinamembers
  • Calibration of the TonoVet and Tono-Pen Vet tonometers in pigs´ eyesmembers
  • Measurement of serum cobalamin and folate concentrations in pigs with commercially assaysmembers
  • Commercially available immunoassays for serum cobalamin and folate in pigsmembers
  • Ultrasound-guided vessel catheterization in adult pigsmembers
  • Association between porcine cytomegalovirus infection and PMWSmembers
  • Suitable antibiotic therapy to treat swine brucellosismembers
  • Pancuronium versus vecuronium in pigs members
  • Haemodynamic effects of pancuronium and vecuronium in pigsmembers
  • Exposure of pigs to lead from contaminated zinc oxide members
  • Conjugated Linoleic Acid and Growth Performance and Losses of Pigletsmembers
  • Introduction of PRRSV via Boar Semen into a PRRSV-Free Countrymembers


  • [ Home ] [ About ] [ Contact / Request ][ Disclaimer ]

    Copyright © 2001-2016 VetContact GmbH
    All rights reserved