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D- and L-lactate in the blood of healthy rabbits
What are the whole blood and serum concentrations of l-lactate and serum concentrations of d-lactate in healthy rabbits? And which of the three methods of analysis for l-lactate measurement compared in this study is the best? Interesting questions which were answered in this prospective study of 25 rabbits.

Concentrations of whole blood l-lactate were measured using a portable analyser and a blood gas analyser.

The remainder of the sample was allowed to clot for centrifugation.

Serum was stored at ‚ąí20¬įC for determination of l- and d- lactate by high-performance liquid chromatography.

D-lactate values by high-performance liquid chromatography were 0‚ÄȬ∑‚ÄČ17‚ÄȬĪ‚ÄČ0‚ÄȬ∑‚ÄČ08 mmol/L.

L-lactate values were 5‚ÄȬ∑‚ÄČ1 (¬Ī2‚ÄȬ∑‚ÄČ1) mmol/L by high-performance liquid chromatography, 6‚ÄȬ∑‚ÄČ9 (¬Ī2‚ÄȬ∑‚ÄČ7) mmol/L with the portable analyser and 7‚ÄȬ∑‚ÄČ1 (¬Ī1‚ÄȬ∑‚ÄČ6) mmol/L with the blood gas analyser.

No significant difference (P‚ÄČ>‚ÄČ0‚ÄȬ∑‚ÄČ05) was found between the two analysers.

Significant difference existed between serum l-lactate values obtained by high-performance liquid chromatography and the whole blood values obtained with the blood gas analyser (P‚ÄČ<‚ÄČ0‚ÄȬ∑‚ÄČ01) and portable analyser (P‚ÄČ<‚ÄČ0‚ÄȬ∑‚ÄČ05).

Serum concentrations of d-lactate in healthy rabbits are in the range of those of other mammals, whereas l-lactate values in healthy rabbits are higher compared with other mammals.

Good correlation was found between the portable and blood gas analysers for whole blood l-lactate measurement in healthy rabbits.


Source: Langlois, I., Planch√©, A., Boysen, S. R., Abeysekara, S. and Zello, G. A. (2014), Blood concentrations of d- and l-lactate in healthy rabbits. Journal of Small Animal Practice, 55: 451‚Äď456. doi: 10.1111/jsap.12247


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SMALL ANIMAL PRACTICE

Aldosterone-¬źproducing adrenocortical carcinoma with myxoid differentiation in a Persian cat
A 10‚Äźyear‚Äźold male neutered Persian cat was presented with an abdominal mass and history of weakness. Blood smear examination found marked elliptocytosis, and serum biochemical analysis revealed hypokalemia, hypochloremia, increased creatine kinase activity, and a high aldosterone concentration. Cytologic examination of the mass revealed neoplastic endocrine cells with moderate criteria of malignancy, favoring adrenocortical neoplasia. A very interesting case report!

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