|D- and L-lactate in the blood of healthy rabbits
What are the whole blood and serum concentrations of l-lactate and serum concentrations of d-lactate in healthy rabbits? And which of the three methods of analysis for l-lactate measurement compared in this study is the best? Interesting questions which were answered in this prospective study of 25 rabbits. |
|Concentrations of whole blood l-lactate were measured using a portable analyser and a blood gas analyser.
The remainder of the sample was allowed to clot for centrifugation.
Serum was stored at ‚ąí20¬įC for determination of l- and d- lactate by high-performance liquid chromatography.
D-lactate values by high-performance liquid chromatography were 0‚ÄČ¬∑‚ÄČ17‚ÄČ¬Ī‚ÄČ0‚ÄČ¬∑‚ÄČ08 mmol/L.
L-lactate values were 5‚ÄČ¬∑‚ÄČ1 (¬Ī2‚ÄČ¬∑‚ÄČ1) mmol/L by high-performance liquid chromatography, 6‚ÄČ¬∑‚ÄČ9 (¬Ī2‚ÄČ¬∑‚ÄČ7) mmol/L with the portable analyser and 7‚ÄČ¬∑‚ÄČ1 (¬Ī1‚ÄČ¬∑‚ÄČ6) mmol/L with the blood gas analyser.
No significant difference (P‚ÄČ>‚ÄČ0‚ÄČ¬∑‚ÄČ05) was found between the two analysers.
Significant difference existed between serum l-lactate values obtained by high-performance liquid chromatography and the whole blood values obtained with the blood gas analyser (P‚ÄČ<‚ÄČ0‚ÄČ¬∑‚ÄČ01) and portable analyser (P‚ÄČ<‚ÄČ0‚ÄČ¬∑‚ÄČ05).
Serum concentrations of d-lactate in healthy rabbits are in the range of those of other mammals, whereas l-lactate values in healthy rabbits are higher compared with other mammals.
Good correlation was found between the portable and blood gas analysers for whole blood l-lactate measurement in healthy rabbits.
Source: Langlois, I., Planch√©, A., Boysen, S. R., Abeysekara, S. and Zello, G. A. (2014), Blood concentrations of d- and l-lactate in healthy rabbits. Journal of Small Animal Practice, 55: 451‚Äď456. doi: 10.1111/jsap.12247
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