|ELISA against circulating anti-asparaginase antibodies in dogs with lymphoid neoplasia
Lymphomas belong to the most common malignant tumours in dogs and they are in most cases very sensitive to chemotherapy. L-aspariginase is one of the commonly used chemotherapeutic substances, but unfortunately resistance to Escherichia coli l-asparaginase in canine lymphoma occurs frequently with repeated administration, a phenomenon often attributed, without substantiation, to the induction of neutralizing antibodies. |
|The objective of this study was to test the hypothesis that treated dogs develop antibodies against the drug, we created an enzyme-linked immunosorbent assay (ELISA) to measure plasma anti-asparaginase immunoglobulin G responses.
Using samples from dogs that had received multiple doses, specific reactivity against l-asparaginase was demonstrated, while naÃ¯ve patients` samples were negative.
The optimized ELISA appeared sensitive, with endpoint titers >1 600 000 in positive control dogs.
Intra- and inter-assay coefficients of variation were 3.6 and 14.5%.
The assay was supported by the observation that ELISA-positive plasma could immunoprecipitate asparaginase activity.
When clinical patients were evaluated, 3/10 dogs developed titers after a single injection; with repeated administration, 4/7 dogs were positive. l-asparaginase antibodies showed reduced binding to the PEGylated drug formulation.
The ELISA should prove useful in investigating the potential correlation of antibody responses with resistance.
Source: Kidd, J. A., Ross, P., Buntzman, A. S. and
Hess, P. R. (2015), Development of an ELISA to detect circulating anti-asparaginase antibodies in dogs with lymphoid neoplasia treated with Escherichia coli l-asparaginase. Veterinary and Comparative Oncology, 13: 77â88. doi: 10.1111/vco.12014
Tell a friend
Send this article
Metastasis of a well differentiated perianal gland tumorPunica granatum associated with hepatotoxicosis in cattleToceranib phosphate (PalladiaÂ®) in canine gastrointestinal stromal tumorsRadioactive iodine uptake in hyperthyroid cats after rh-TSHHypoechoic tissue changes in dogs with malignant prostatic lymphomaEmphysematous gastritis in dogs and catsPrimary pulmonary histiocytic sarcoma in dogsDetermining prognosis in canine sepsis Correlation of plasma and tear glucose, creatinine and urea nitrogen in catsPerineal hernias in dogs - always a bilateral problem?Pharmacokinetic of gabapentin in catsFollicular development of canine ovaries stimulated by eCG plus hCG