Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Privacy Policy  
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Swine Practice /     
 
Pregnancy diagnosis in miniature pigs by fecal ELISA
Miniature pigs are more and more seen as pets in different countries. A Japanese group evaluated a new fecal ELISA test from pregnancy diagnosis at day 27-29.

The objectives of the present study were to measure oestrone derivatives [oestrone, oestrone sulphate (E1S) and oestrone glucuronide] in sow faeces by direct enzyme-linked immunosorbent assay (ELISA) and to explore the changes of oestrone derivative profile in faeces of miniature pig for demonstrating the possibility of pregnancy diagnosis.

Faecal samples (1.5 g) were put into 6 ml of buffer, shaken and centrifuged. Then supernatant was added to the wells of multi-plate without extraction and incubated with anti-oestrone antibody and horseradish peroxidase-labelled oestrone. Standard solution was prepared at various concentrations of E1S. Sensitivity was estimated as 0.035 ng/ml (0.14 ng/g). Intra- and inter-assay coefficient variations were 3.5-7.7% and 10.9-15.3%, respectively. When 1-5 ng/ml E1S were added to a faecal solution, recovery rates ranged between 80.0 and 103.3%. There is a temporal increase in the E1S equivalent concentration of miniature pig faeces from day 25 to 31 after mating. From day 35 to 70, the E1S equivalent concentrations remained low. Thereafter its concentrations increased again towards farrowing.

On day 27 and 29 after mating pregnancy diagnosis by the faecal E1S equivalent concentrations agreed with the results of farrowing (seven of seven animals).

These results suggest that the present direct ELISA is practical and suitable as a routine assay for measuring the faecal concentration of oestrone derivatives and that this assay might be usable for pregnancy diagnosis in sows at day 27-29.


Source: N Isobe and T Nakao (2004): Pregnancy Diagnosis in Miniature Pig by Direct ELISA of Oestrone Derivatives in Faeces. In: Reproduction in Domestic Animals 39 (1), 48-51.





Tell a friend   |   Print version   |   Send this article

SWINE PRACTICE

Pasteurella multocida diversity in poultry and pigs in Australiamembers
This recently published study investigates the genotype and diversity of Pasteurella multocida present in pig herds and determines the extent of overlap with isolates from poultry flocks in Australia. Do the isolates vary? A very interesting study not only for Australia.

  • Enrofloxacin and the porcine livermembers
  • Outbreak of PEDV in Italymembers
  • Actinobacillus rossii as cause of abortion in a piglet members
  • Association between genome and aggression in group-housed pigsmembers
  • Oral fluid and plasma oxytetracycline concentrations after intramuscular administration in pigsmembers
  • Pharmacokinetics and behavioral effects of a liquid transdermal fentanyl solution in pigsmembers
  • Beta hydroxy beta methyl butyrate and the muscle fibre composition in growing pigsmembers
  • L-carnitine as a feed additive in pigs and poultrymembers
  • PCV-3 in pig serum samples from Spainmembers
  • Oral fluid and plasma oxytetracycline concentrations after i.m. administration in pigsmembers
  • Novel highly pathogenic porcine reproductive and respiratory syndrome virus in Chinamembers
  • Calibration of the TonoVet and Tono-Pen Vet tonometers in pigs´ eyesmembers


  • [ Home ] [ About ] [ Contact / Request ] [ Privacy Policy ]

    Copyright © 2001-2018 VetContact GmbH
    All rights reserved