Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Privacy Policy  
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Swine Practice /     
 
Susceptibility of Porcine Embryos to Classical Swine Fever Virus
A very interesting question: Is there a difference in suspectibility of embryos to classic swine fever virus, if they are either in vitro or in vivo produced? It seems so, as this brandnew German study shows. Still further in vivo tests are necessary to confirm these results.

The objective of this study was to investigate the susceptibility of in vivo- and in vitro-produced (IVP) porcine embryos to classical swine fever virus (CSFV).

IVP zona pellucida (ZP)-intact porcine embryos (n = 721) were co-cultured with CSFV for 120 h. After washing according to the International Embryo Transfer Society guidelines (without trypsin) and transferring embryos to CSFV-susceptible porcine kidney cells (PK15 cell line), no virus was isolated.

However, when 88 IVP ZP-intact porcine embryos were co-cultured with CSFV for only 48 h before being transferred to PK15 cells, virus was isolated in three of six replicates.

Similarly, 603 in vivo-produced porcine embryos were co-cultured with CSFV for 120 h.

Subsequently, CSFV was isolated in eight of 50 groups (16%) and the ability of these to form a blastocyst was significantly reduced when compared with the control group (68.2 ± 19.9% vs 81.9 ± 9.7%; p 0.001). I

n contrast, the development of CSFV-exposed IVP porcine embryos was not affected when compared with control embryos (19.1 ± 10.8% vs 18.9 ± 10.6%; p 0.05).

After removal of the ZP of IVP embryos and subsequent co-culture with CSFV, the virus was isolated from all groups of embryos.

These data suggest that virus replication had occurred in the embryonic cells.

In conclusion, data indicate that in vivo- and in vitro-produced ZP-intact porcine embryos differ in their susceptibility to CSFV.

Hatched or micro-manipulated embryos may increase the risk of transmission of CSFV by embryo transfer, which has to be confirmed by in vivo tests under isolation conditions.


Source: Schüürmann, E, Flögel-Niesmann, G, Mönnig, V & Rath, D (2005): Susceptibility of In Vivo- and In Vitro-produced Porcine Embryos to Classical Swine Fever Virus. In: Reproduction in Domestic Animals 40 (5), 415-421.





Tell a friend   |   Print version   |   Send this article

SWINE PRACTICE

Pasteurella multocida diversity in poultry and pigs in Australiamembers
This recently published study investigates the genotype and diversity of Pasteurella multocida present in pig herds and determines the extent of overlap with isolates from poultry flocks in Australia. Do the isolates vary? A very interesting study not only for Australia.

  • Enrofloxacin and the porcine livermembers
  • Outbreak of PEDV in Italymembers
  • Actinobacillus rossii as cause of abortion in a piglet members
  • Association between genome and aggression in group-housed pigsmembers
  • Oral fluid and plasma oxytetracycline concentrations after intramuscular administration in pigsmembers
  • Pharmacokinetics and behavioral effects of a liquid transdermal fentanyl solution in pigsmembers
  • Beta hydroxy beta methyl butyrate and the muscle fibre composition in growing pigsmembers
  • L-carnitine as a feed additive in pigs and poultrymembers
  • PCV-3 in pig serum samples from Spainmembers
  • Oral fluid and plasma oxytetracycline concentrations after i.m. administration in pigsmembers
  • Novel highly pathogenic porcine reproductive and respiratory syndrome virus in Chinamembers
  • Calibration of the TonoVet and Tono-Pen Vet tonometers in pigs´ eyesmembers


  • [ Home ] [ About ] [ Contact / Request ] [ Privacy Policy ]

    Copyright © 2001-2018 VetContact GmbH
    All rights reserved