Given the pathogenic role of BPV-1 and BPV-2 in sarcoid disease, we suggest that intralesional viral DNA concentration may reflect the degree of affection.
Severity of disease was addressed by recording the tumour growth kinetics, lesion number and tumour type for 37 sarcoid-bearing horses and one donkey. ´
Viral load was estimated via quantitative real-time PCR (qPCR) of the E2, E5, L1 and L2 genes from the BPV-1/-2 genome for one randomly selected lesion per horse and correlated with disease severity.
Results: Quantitative PCR against E2 identified viral DNA concentrations ranging from 0556 copies/tumour cell.
Of 16 horses affected by quiescent, slowly growing single tumours or multiple mild-type lesions, 15 showed a viral load up to 1.4 copies per cell.
In stark contrast, all equids (22/22) bearing rapidly growing and/or multiple aggressive sarcoids had a viral load between 3 and 569 copies per cell. Consistent results were obtained with qPCR against E5, L1 and L2.
Conclusions: While tumours of the same clinical type carried variable virus load, confirming that viral titre does not determine clinical appearance, we identified a highly significant correlation between intralesional viral load and disease severity.
Potential relevance: The rapid determination of BPV viral load will give a reliable marker for disease severity and may also be considered when establishing a therapeutic strategy.
Source: R. HARALAMBUS, J. BURGSTALLER, J. KLUKOWSKA-RÖTZLER, R. STEINBORN, S. BUCHINGER, V. GERBER and S. BRANDT (2010): Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease. In: Equine Veterinary Journal
Volume 42 Issue 4, Pages 327 - 331
Published Online: 15 Apr 2010
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