Twenty-five healthy dogs, including 9 laboratory beagle dogs, and 22 client-owned dogs with GBM were enrolled.
Serum leptin concentration was determined in blood samples of all dogs by ELISA.
Canine gallbladder samples were collected from 9 dogs with GBM that underwent surgery for therapeutic purposes and from 9 healthy laboratory beagle dogs as a normal control group.
Samples were analyzed for leptin and leptin receptor mRNA by real-time polymerase chain reaction.
Serum leptin concentration was significantly higher in dogs with GBM than in healthy dogs (medians of 7.03 and 2.18 ng/mL, respectively; P < .001).
Patients with GBM that had undergone surgery had significantly higher serum leptin concentrations than those that had not (medians of 12.2 and 4.09 ng/mL, respectively; P = .001).
However, no difference in serum leptin concentration was found between dogs with GBM with or without endocrinopathies.
The mRNA expression levels of leptin and its receptor were significantly increased in the gallbladder tissues of dogs with GBM.
Dysregulation of leptin might be involved in the pathophysiology of GBM, and leptin concentrations might be associated with GBM severity.
Source: Lee, S., Kweon, O.-K. and Kim, W.H. (2016), Increased Leptin and Leptin Receptor Expression in Dogs With Gallbladder Mucocele. Journal of Veterinary Internal Medicine. doi: 10.1111/jvim.14612
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