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Mycobacterium bovis in a cattle herd
Cattle are the host and main reservoir of the etiologic agent of bovine tuberculosis, Mycobacterium bovis; although other mammalian species, including humans, are susceptible. The tuberculin test and/or slaughterhouse surveillance is the diagnostic method used by control programs all around the world to control and eradicate the disease. Are there better alternatives?

In order to compare different tuberculosis diagnostic tests and to reach disease confirmation, a study was performed in a group of 14 steers of Friesian breed, reacting positively to tuberculin test.

Three ante-mortem assays were performed according to the type of sample: the gamma interferon (IFN-¦Ã) test (which quantifies the release of this cytokine by sensitized lymphocytes in whole blood in response to purified protein derivative (PPD) and recombinant ESAT-6 and CFP10 proteins); PCR and bacteriologic culture from nasal swab and intradermal tuberculin test.

These assays were taken at different times to assess the evolution of clinical parameters. Post-mortem examination showed macroscopic and microscopic tuberculosis lesions with acid-fast bacillus and positive cultures.

By spoligotyping, we observed that all the isolates showed the same pattern.

The positive results based on comparison to lesions observed ranged from 58% to 75% for the IFN-¦Ã assays, to 72% for cultures, and ranged from 50% to 90% for PCR in nasal swabs.

In conclusion, in a herd infected by the same strain, ante-mortem direct and immune-diagnostic parameters change, suggesting that several tests are needed for a faster control of infection at herd level.



Source: V. Meikle, M. Schneider, G. Azenzo, M. Zum¨¢rraga, G. Magnano, A. Cataldi (2007): Individual Animals of a Cattle Herd Infected with the Same Mycobacterium bovis Genotype Shows Important Variations in Bacteriological, Histopathological and Immune Response Parameters. In: Zoonoses and Public Health 54 (2), 86¨C93.


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BOVINE

Subclinical ketosis in dairy cows: prevalence and risk factorsmembers
Subclinical ketosis is commonly seen especially in dairy cows and grazing systems aand can become a serious and sometimes life threatening metabolic problem. The purpose of this study was to estimate the prevalence of Subclinical ketosis (SCK) between 4 and 19 days in milk (DIM) in a grazing production system and investigate the importance of potential risk factors for SCK.

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  • Tulathromycin versus tildipirosin in experimental Mycoplasma bovis infection in calvesmembers
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  • Long-term Effects of Pyrethrin and Cyfluthrin on Bull Reproductive Parametersmembers
  • Arthroscopy of the bovine antebrachiocarpal and middle carpal jointmembers
  • Degree of corneal anaesthesia after topical application of various drugsmembers
  • Improvement of the outcome in recumbent dairy cattlemembers
  • Secondary damage in downer cows - an underestimated problemmembers


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