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Severe Trichophytosis outbreak in flocks of green iguanas
Trichophyton mentagrophytes is commmonly involved in dermatophytoses in small animals, and the main source of infection are small mammals. This case report describes the clinical, mycological, histopathological and molecular findings in green iguanas (Iguana iguana) affected with severe dermatophytosis in selected flocks near Tehran, Iran.



Samples were collected from the scales of skin lesions and tested with standard mycological methods and dermatophyte-specific PCR amplification analysis using the primer pair for the chitin synthase 1(CHS1) gene.

Results: All iguanas were definitively diagnosed with dermatophytosis using both traditional and molecular diagnostic methods.

PCR fingerprinting profiles using simple repetitive (GACA)4 primers showed that all diagnosed iguanas had the same pattern profile.

Intraspecific variability was not observed for these isolates. Oligonucleotide sequencing of CHS1 gene PCR products confirmed Trichophyton mentagrophytes var. interdigitale as the infectious agent.

Clinical Significance: These results suggest that (GACA)4-based PCR has utility both as a simple and rapid method for identification of dermatophyte species and for differentiation of T. mentagrophytes variants.


Source: Khosravi, A. R., Shokri, H., Rostami, A., Tamai, I. A., Erfanmanesh, A. and Memarian, I. (2012), Severe dermatophytosis due to Trichophyton mentagrophytes var. interdigitale in flocks of green iguanas (Iguana iguana). Journal of Small Animal Practice, 53: 286–291. doi: 10.1111/j.1748-5827.2011.01217.x




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SMALL ANIMAL PRACTICE

Reference intervals for blood parameters in Shetland Sheepdogsmembers
Several breeds have physiological peculiarities that induce variations in reference intervals (RIs) compared with the general canine population. Shetland sheepdogs (SSs) are reported to be more predisposed to different diseases (eg, hyperlipidemia, gallbladder mucocele, and hypothyroidism). Consequently, a breed‐specific approach is more often required. Thus, the aim of this study was to determine whether the RIs of the general canine population could be applied to that of SSs, and to generate breed‐specific RIs, where appropriate.

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