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Severe Trichophytosis outbreak in flocks of green iguanas
Trichophyton mentagrophytes is commmonly involved in dermatophytoses in small animals, and the main source of infection are small mammals. This case report describes the clinical, mycological, histopathological and molecular findings in green iguanas (Iguana iguana) affected with severe dermatophytosis in selected flocks near Tehran, Iran.



Samples were collected from the scales of skin lesions and tested with standard mycological methods and dermatophyte-specific PCR amplification analysis using the primer pair for the chitin synthase 1(CHS1) gene.

Results: All iguanas were definitively diagnosed with dermatophytosis using both traditional and molecular diagnostic methods.

PCR fingerprinting profiles using simple repetitive (GACA)4 primers showed that all diagnosed iguanas had the same pattern profile.

Intraspecific variability was not observed for these isolates. Oligonucleotide sequencing of CHS1 gene PCR products confirmed Trichophyton mentagrophytes var. interdigitale as the infectious agent.

Clinical Significance: These results suggest that (GACA)4-based PCR has utility both as a simple and rapid method for identification of dermatophyte species and for differentiation of T. mentagrophytes variants.


Source: Khosravi, A. R., Shokri, H., Rostami, A., Tamai, I. A., Erfanmanesh, A. and Memarian, I. (2012), Severe dermatophytosis due to Trichophyton mentagrophytes var. interdigitale in flocks of green iguanas (Iguana iguana). Journal of Small Animal Practice, 53: 286–291. doi: 10.1111/j.1748-5827.2011.01217.x




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SMALL ANIMAL PRACTICE

Patient-specific facemask to facilitate brain biopsymembers
The objective of this pilot study was to describe the application and first preliminary data of a novel MRI and CT compatible patient-specific facemask for stereotactic brain biopsy of intracranial lesions in dogs. Five client-owned dogs presenting for neurological deficits consistent with forebrain disease were included in the study. All dogs had MRI findings consistent with an intracranial lesion. But biopsies in this region are not easy to obtain. Does an individual face mask help?

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