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EDTA versus lithium heparin samples to measure PCV and TPr
It is assumed that lithium heparin (LiH) and potassium ethylenediaminetetraacetic acid (EDTA) can be used interchangeably to obtain packed cell volume (PCV) and total protein by refractometry (TPr). Is this really true and can those values be compared with laboratory derived haematocrit (Hct) and total protein (TP) concentration, respectively, in canine blood samples?

Blood samples taken in LiH and EDTA were manually assessed for PCV and TPr. Results were correlated to Hct and TP.

238 EDTA and corresponding serum/LiH samples were obtained.

There was excellent correlation but statistically significant difference between LiH and EDTA PCV (n=43).

LiH and EDTA TPr (n=43) were excellently correlated without significant difference. PCV and Hct (n=176) were excellently correlated without significant difference.

LiH (n=105) and serum (n=133) TP was respectively fairly or well correlated with TPr but with significant differences.

An increase in cholesterol of 1 mmol/L was associated with a mean independent increase in TPr of approximately 1 g/L.

LiH and EDTA can be used interchangeably for TPr.

Although TPr and serum/plasma TP were correlated, there were statistically significant differences that could impact on clinical decision making.

TPr is increased by cholesterol but this alone could not account for the magnitude of the difference observed.



Source: Tamborini, A., Papakonstantinou, S., Brown, A., Brennan, S., Shiel, R. E., Mills, J. N. and Mooney, C. T. (2014), Comparison of manual and laboratory PCV and total protein using EDTA and lithium heparin canine samples. Journal of Small Animal Practice, 55: 258–264. doi: 10.1111/jsap.12198



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