In the present study, we show by Western blot in 4 sarcoid samples and 3 normal equine skin samples that the PDGFâ-r is more phosphorylated in sarcoid tissue than in normal skin (P < .001).
Furthermore, the physical interaction between the activated receptor and the 85-kDa regulatory subunit (p85) of phosphatidylinositol-3-kinase (PI3K) is shown by coimmunoprecipitation.
The PI3K–AKT–cyclin D3 molecular pathway downstream to the activation of the PDGFâ-r is shown to be expressed, and the amount of the investigated molecules is higher than normal (P < .001), suggesting an activation of these effectors in sarcoids.
Further, we demonstrate that phospho-JNK and phospho-JUN are more expressed in sarcoids than in normal skin.
Our results provide new insights into the pathogenesis of equine sarcoids and support the validity of this in-vivo model to further characterize the molecular pathways underlying BPV E5–induced carcinogenesis.
Source: G. Borzacchiello, S. Mogavero, G. De Vita, S. Roperto, L.Della Salda and F. Roperto (2009): Activated Platelet-Derived Growth Factor â Receptor Expression, PI3K-AKT Pathway Molecular Analysis, and Transforming Signals in Equine Sarcoids. In: Vet Pathol 46:589-597 (2009)
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