|Fifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food-responsive diarrhea (FRD) were included in this prospective study.
mRNA expression of COX-2, 5-LO, IL-1b, IL-4, IL-6, TNF, IL-10 and TFG-Î² was evaluated by quantitative real-time RT-PCR in duodenal and colonic biopsies before and after treatment.
COX-2 expression in the colon was significantly higher in IBD and FRD before and after treatment (all P < .01).
IL-1b was higher in FRD in the duodenum after treatment (P = .021).
TGF-Î² expression was significantly higher in the duodenum of HCD compared to FRD/IBD before treatment (both P < .001) and IBD after treatment (P = .012).
There were no significant differences among groups and within groups before and after treatment for IL-4, IL-6, TNF, and IL-10.
There was a significant correlation between COX-2 and IL-1b in duodenum and colon before treatment in FRD and IBD, whereas 5-LO correlated better with IL-6 and TNF.
IL-10 and TGF-Î² usually were correlated.
COX-2 is upregulated in IBD and FRD, whereas IL-1b and TGF-Î² seem to be important pro- and anti-inflammatory cytokines, respectively.
The use of dual COX/5-LO inhibitors could be an interesting alternative in the treatment of CCE.
Source: Dumusc, S.D., Ontsouka, E.C., Schnyder, M., Hartnack, S., Albrecht, C., Bruckmaier, R.M. and Burgener, I.A. (2014), Cyclooxygenase-2 and 5-Lipoxygenase in Dogs with Chronic Enteropathies. Journal of Veterinary Internal Medicine, 28: 1684â1691. doi: 10.1111/jvim.12463
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