Home
http://www.virbac.fr/ http://www.boehringer-ingelheim.com/ http://www.novartis.com/ http://www.animalhealth.bayerhealthcare.com/
vetcontact
Vetrinär
Tiermedizin
  WELCOME  
vetcontact
Vetrinär
Tiermedizin
  Privacy Policy  
  Home  
  Login / Newsletter  
vetcontact
Vetrinär
Tiermedizin
  CONTACTS  
vetcontact
Vetrinär
Tiermedizin
  Classifieds  
  New Products  
  VetCompanies  
  VetSchools  
vetcontact
Vetrinär
Tiermedizin
  PROFESSION  
vetcontact
Vetrinär
Tiermedizin
  Edutainment  
  VetAgenda  
  Presentations  
  Posters  
  ESAVS  
  Specialisation  
vetcontact
Vetrinär
Tiermedizin
  INSIGHT  
vetcontact
Vetrinär
Tiermedizin
  Congress News  
  Picture Galleries  
vetcontact
Vetrinär
Tiermedizin
  PRODUCTS  
vetcontact
Vetrinär
Tiermedizin
  Bayer  
  Boehringer Ing.  
  Novartis  
  Virbac

 
  Simply book for less...  
    

Bovine    Equine    Small Animal Practice    Swine Practice    Articles    Vetjournal    
deutsch english español polski francais
Home / WELCOME / Archiv / Small Animal Practice /     
 
ELISA against circulating anti-asparaginase antibodies in dogs with lymphoid neoplasia
Lymphomas belong to the most common malignant tumours in dogs and they are in most cases very sensitive to chemotherapy. L-aspariginase is one of the commonly used chemotherapeutic substances, but unfortunately resistance to Escherichia coli l-asparaginase in canine lymphoma occurs frequently with repeated administration, a phenomenon often attributed, without substantiation, to the induction of neutralizing antibodies.

The objective of this study was to test the hypothesis that treated dogs develop antibodies against the drug, we created an enzyme-linked immunosorbent assay (ELISA) to measure plasma anti-asparaginase immunoglobulin G responses.

Using samples from dogs that had received multiple doses, specific reactivity against l-asparaginase was demonstrated, while naïve patients` samples were negative.

The optimized ELISA appeared sensitive, with endpoint titers >1 600 000 in positive control dogs.

Intra- and inter-assay coefficients of variation were 3.6 and 14.5%.

The assay was supported by the observation that ELISA-positive plasma could immunoprecipitate asparaginase activity.

When clinical patients were evaluated, 3/10 dogs developed titers after a single injection; with repeated administration, 4/7 dogs were positive. l-asparaginase antibodies showed reduced binding to the PEGylated drug formulation.

The ELISA should prove useful in investigating the potential correlation of antibody responses with resistance.



Source: Kidd, J. A., Ross, P., Buntzman, A. S. and
Hess, P. R. (2015), Development of an ELISA to detect circulating anti-asparaginase antibodies in dogs with lymphoid neoplasia treated with Escherichia coli l-asparaginase. Veterinary and Comparative Oncology, 13: 77–88. doi: 10.1111/vco.12014


Tell a friend   |   Print version   |   Send this article

SMALL ANIMAL PRACTICE

Reference intervals for blood parameters in Shetland Sheepdogsmembers
Several breeds have physiological peculiarities that induce variations in reference intervals (RIs) compared with the general canine population. Shetland sheepdogs (SSs) are reported to be more predisposed to different diseases (eg, hyperlipidemia, gallbladder mucocele, and hypothyroidism). Consequently, a breedâ€specific approach is more often required. Thus, the aim of this study was to determine whether the RIs of the general canine population could be applied to that of SSs, and to generate breedâ€specific RIs, where appropriate.

  • Metastasis of a well differentiated perianal gland tumor
  • Punica granatum associated with hepatotoxicosis in cattlemembers
  • Toceranib phosphate (Palladia®) in canine gastrointestinal stromal tumorsmembers
  • Radioactive iodine uptake in hyperthyroid cats after rh-TSHmembers
  • Hypoechoic tissue changes in dogs with malignant prostatic lymphomamembers
  • Emphysematous gastritis in dogs and catsmembers
  • Primary pulmonary histiocytic sarcoma in dogsmembers
  • Determining prognosis in canine sepsis members
  • Correlation of plasma and tear glucose, creatinine and urea nitrogen in catsmembers
  • Perineal hernias in dogs - always a bilateral problem?members
  • Pharmacokinetic of gabapentin in catsmembers
  • Follicular development of canine ovaries stimulated by eCG plus hCGmembers


  • [ Home ] [ About ] [ Contact / Request ] [ Privacy Policy ]

    Copyright © 2001-2018 VetContact GmbH
    All rights reserved