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Dermatology Home DERMATOLOGY
IN VITRO MODEL OF MICROSPORUM CANIS DERMATOPHYTOSIS
Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant in vivo studies particularly difficult to perform. At the University of L├╝ttich a group has developed an in vitro model of M. canis dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis.

The aim of this study was to develop a new in vitro model of M. canis dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis, and to investigate its relevance in studying the host-pathogen relationship.

Histological analysis of reconstructed interfollicular feline epidermis (RFE) revealed a fully differentiated epidermis.

A proliferation assay showed replicating cells only in the basal layer, indicating that RFE is a well-stratified living tissue, leading to the formation of a horny layer.

Histopathological analysis of RFE infected by M. canis arthroconidia revealed that the fungus invades the stratum corneum and produces SUB3, a keratinase implicated in the infectious process.

In view of these results, an M. canis dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural M. canis feline infections.



Source: Tabart J, Baldo A, Vermout S, Nusgens B, Lapiere C, Losson B, Mignon B. (2007): Reconstructed interfollicular feline epidermis as a model for Microsporum canis dermatophytosis. In: J Med Microbiol. 2007 Jul;56(Pt 7):971-5.

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